Complete this section for all typing done by DNA based methods. Examples of HLA typing by DNA technology may include: sequence-specific primer (SSP), sequence-specific oligonucleotide probe (SSOP), and sequence-based typing (SBT).
DNA technology can be used to type for a single allele, combinations of alleles (allele strings), or a “generic” allele designation similar to a serologic typing result. For this reason, the number of digits reported, as well as the number of alleles, will vary.
Laboratories may use “ / ” , “ – ” or a combination of numbers and letters on the typing report as a shorthand notation for the results. Transcribe the information onto the form as directly as possible. The letters, called allele codes, will be 1 or more characters in length and represent a combination of possible alleles at a locus. The same allele combination may be reported several different ways (e.g., DRB1*01:01 or 01:02, DRB1*01:01/01:02, DRB1*01:01/02, or DRB1*01:AB).
There will be two alleles reported for each locus, unless the individual is presumed homozygous (i.e., carries two copies of the same allele) at a locus. Transcribe the first allele designation in the first box, and the second allele designation in the second box. If the person is homozygous, leave the second box blank.
Question 2: Was documentation submitted to the CIBMTR (e.g., lab report)?
Indicate if a copy of the HLA typing report is attached. Use the “Add Attachment” feature to attach a copy of the HLA typing report in FormsNet3SM. Attaching a copy of the laboratory report assists in confirming the reporting of HLA typing and reduces the need for later data queries.
Class I
Questions 3-4: Locus A
Indicate whether the allele designations at HLA-A are Known or Unknown. If Known, report the first A* allele and second A* allele designations; report a single allele, a string of alleles, or an allele code.
If Unknown, then the A antigens defined by serologic typing are required to be answered below.
Questions 5-6: Locus B
Indicate whether the allele designations at HLA-B are Known or Unknown. If Known, report the first B* allele and second B* allele designations; report a single allele, a string of alleles, or an allele code.
If Unknown, then the B antigens defined by serologic typing are required to be answered below.
Questions 7-8: Locus C
Indicate whether the allele designations at HLA-C are Known or Unknown. If Known, report the first C* allele and second C* allele designations; report a single allele, a string of alleles, or an allele code.
Class II
Questions 9-10: Locus DRB1
Indicate whether the allele designations at HLA-DRB1 are Known or Unknown. If Known, report the first DRB1* allele and second DRB1* allele designations; report a single allele, a string of alleles, or an allele code.
Class II (Optional)
Questions 11-12: Locus DRB3
Indicate whether the allele designations at HLA-DRB3 are Known or Unknown. If Known, report the first DRB3* allele and second DRB3* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 13-14: Locus DRB4
Indicate whether the allele designations at HLA-DRB4 are Known or Unknown. If Known, report the first DRB4* allele and second DRB4* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 15-16: Locus DRB5
Indicate whether the allele designations at HLA-DRB5 are Known or Unknown. If Known, report the first DRB5* allele and second DRB5* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 17-18: Locus DQB1
Indicate whether the allele designations at HLA-DQB1 are Known or Unknown. If Known, report the first DQB1* allele and second DQB1* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 19-20: Locus DPB1
Indicate whether the allele designations at HLA-DPB1 are Known or Unknown. If Known, report the first DPB1* allele and second DPB1* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 21-22: Locus DQA1
Indicate whether the allele designations at HLA-DQA1 are Known or Unknown. If Known, report the first DQA1* allele and second DQA1* allele designations; report a single allele, a string of alleles, or an allele code.
Questions 23-24: Locus DPA1
Indicate whether the allele designations at HLA-DPA1 are Known or Unknown. If Known, report the first DPA1* allele and second DPA1* allele designations; report a single allele, a string of alleles, or an allele code.
Section Updates:
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