Q2 – 23: HLA Typing by DNA Technology

Complete this section for all typing done by DNA based methods. Examples of HLA typing by DNA technology may include: sequence-specific primer (SSP), sequence-specific oligonucleotide probe (SSOP), and sequence-based typing (SBT).

DNA technology can be used to type for a single allele, combinations of alleles (allele strings), or a “generic” allele designation similar to a serologic typing result. For this reason, the number of digits reported, as well as the number of alleles, will vary.

Laboratories may use “ / ” , “ – ” or a combination of numbers and letters on the typing report as a shorthand notation for the results. Transcribe the information onto the form as directly as possible. The letters, called allele codes, will be 1 or more characters in length and represent a combination of possible alleles at a locus. The same allele combination may be reported several different ways (e.g., DRB1*01:01 or 01:02, DRB1*01:01/01:02, DRB1*01:01/02, or DRB1*01:AB).

There will be two alleles reported for each locus, unless the individual is presumed homozygous (i.e., carries two copies of the same allele) at a locus. Transcribe the first allele designation in the first box, and the second allele designation in the second box. If the person is homozygous, leave the second box blank.

*Attaching HLA Report
CIBMTR strongly encourages centers to attach a copy of the HLA report in FormsNet3^SM.

Class I

Questions 2 – 3: Locus A

Indicate whether the allele designations at HLA-A are Known or Unknown. If Known, report the first A* allele and second A* allele designations; report a single allele, a string of alleles, or an allele code.

If Unknown, then the A antigens defined by serologic typing are required to be answered below.

Questions 4 – 5: Locus B

Indicate whether the allele designations at HLA-B are Known or Unknown. If Known, report the first B* allele and second B* allele designations; report a single allele, a string of alleles, or an allele code.

If Unknown, then the B antigens defined by serologic typing are required to be answered below.

Questions 6 – 7: Locus C

Indicate whether the allele designations at HLA-C are Known or Unknown. If Known, report the first C* allele and second C* allele designations; report a single allele, a string of alleles, or an allele code.

Class II

Questions 8 – 9: Locus DRB1

Indicate whether the allele designations at HLA-DRB1 are Known or Unknown. If Known, report the first DRB1* allele and second DRB1* allele designations; report a single allele, a string of alleles, or an allele code.

*Class II Optional Alleles
DRB3, DRB4, DRB5, DQB1, DPB1, DQ41, DPA1 are optional; however, if this information is available from the lab, report the allele information.

Class II (Optional)

Questions 10 – 11: Class II Locus DRB3

Indicate whether the allele designations at HLA-DRB3 are Known or Unknown. If Known, report the first DRB3* allele and second DRB3* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 12 – 13: Locus DRB4

Indicate whether the allele designations at HLA-DRB4 are Known or Unknown. If Known, report the first DRB4* allele and second DRB4* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 14 – 15: Locus DRB5

Indicate whether the allele designations at HLA-DRB5 are Known or Unknown. If Known, report the first DRB5* allele and second DRB5* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 16 – 17: Locus DQB1

Indicate whether the allele designations at HLA-DQB1 are Known or Unknown. If Known, report the first DQB1* allele and second DQB1* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 18 – 19: Locus DPB1

Indicate whether the allele designations at HLA-DPB1 are Known or Unknown. If Known, report the first DPB1* allele and second DPB1* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 20 – 21: Locus DQA1

Indicate whether the allele designations at HLA-DQA1 are Known or Unknown. If Known, report the first DQA1* allele and second DQA1* allele designations; report a single allele, a string of alleles, or an allele code.

Questions 22 – 23: Locus DPA1

Indicate whether the allele designations at HLA-DPA1 are Known or Unknown. If Known, report the first DPA1* allele and second DPA1* allele designations; report a single allele, a string of alleles, or an allele code.

Section Updates:

Question Number	Date of Change	Add/Remove/Modify	Description	Reasoning (If applicable)
.	.	.	.	.

Copyright © 2004-2025 The Medical College of Wisconsin, Inc. and NMDP. All Rights Reserved.
CIBMTR® (Center for International Blood and Marrow Transplant Research) is a research collaboration between the Medical College of Wisconsin and NMDP.