RNA Samples:
Send total RNA in deionized water or any elution buffer at a minimum concentration of 5 ng/µl. Deep immune repertoire profiling requires 100 ng of RNA purified from lymphocyte-rich samples. Using less RNA (e.g., 5-20ng) will reduce the efficiency of detection of low-medium abundant clonotypes. More information on sensitivity of clonotype detection can be found in Section 2.4.
Please refer to the RNA preparation protocol for detailed guidelines based on sample type.
DNA Samples:
Send DNA in deionized water or in elution buffer without EDTA (elution buffers with 1mM EDTA could inhibit PCR reaction). Deep immune repertoire profiling requires at least 2 µg of gDNA (with a minimum concentration of 50 ng/µl) purified from lymphocyte-rich samples. For smaller samples, send 250-500 ng of DNA at a minimum concentration of 5 ng/µl. More information on sensitivity of clonotype detection can be found in Section 2.4.
Please refer to the DNA preparation Protocol for detailed guidelines based on sample type.
Shipping Instructions:
- Put the sample in an eppendorf test-tube with # 1-96 labels (attach more information in sample submission form).
- Put tubes in a cardboard box (containing grid), close the lid, and tape the box.
- Ship samples on dry ice for international and blue ice for local shipments.
Ship Samples To:
Attn: Alex Chenchik
Driver Map AIR Service
Cellecta, Inc.
320 Logue Ave
Mountain View, CA 94043
Tel: 650-938-3910
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