Cellecta, Inc.
DriverMap™ Targeted Expression Profiling Kits
Table of Contents
Cellecta, Inc.
  • DriverMap™ Targeted Expression Profiling Kits
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. DriverMap™ Targeted Expression Profiling Assay
  • 2. Materials Required
    • 2.1. Kit Components
    • 2.2. Additional Required Materials
  • 3. Sample Preparation
    • 3.1. RNA Preparation
    • 3.2. DirectCell™ Oligo-dT Capture Protocol
  • 4. cDNA Synthesis and Amplification
    • 4.1. cDNA Synthesis
    • 4.2. Forward Gene-Specific Primer Extension
    • 4.3. Reverse Gene-Specific Primer Extension
    • 4.4. PCR with Anchor Primers
    • 4.5. PCR with Indexed Primers
  • 5. NGS Prep and Sequencing
    • 5.1. Quantify and Combine Samples for NGS
    • 5.2. Purification of the Amplified Indexed Libraries
    • 5.3. Next-Gen Sequencing (NGS)
  • 6. Data Processing and Analysis
    • 6.1. Extract Sequencing Read Data
    • 6.2. Normalize Sample Reads
  • 7. Appendices
    • 7.1. Appendix A. Tips and Troubleshooting
    • 7.2. Appendix B. Sequence of Amplicon after Index Primer PCR
    • 7.3. Appendix C. Forward and Reverse Index Combinations
    • 7.4. Appendix D. Housekeeping Control Genes
    • 7.5. Appendix E: Sample Data for T/B Marker Assay
  • 8. Technical Support
  • 9. Contact Us
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7. Appendices

A. Tips and Troubleshooting
B. Sequence of Amplicon after Index Primer PCR
C. Forward and Reverse Index Combinations
D. Housekeeping Control Genes


Last modified: 8 August 2019
6.2. Normalize Sample Reads
7.1. Appendix A. Tips and Troubleshooting

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