5. NGS Prep and Sequencing

After amplification, the samples may be stored at 4°C until ready to sequence. The product from the PCR with Indexed Primers step contains the P5 and P7 sequences for Next-Gen Sequencing (NGS) on Illumina instruments. The protocols in this section provide instructions to normalize the amount of each PCR product to obtain similar reads for each sample, to clean up samples before loading onto the instrument, and guidelines for sequencing and data analysis.