DriverMap Targeted Expression Profiling Kits contain enough reagents for the preparation of NGS samples starting from total RNA from blood, tissue, cells, or other biological samples, including Positive Control RNA.
Box 1: Kit Components
| Component | Description | Cap Color | Conc. | Volume (24 rxn)* |
Volume (96 rxn) |
|---|---|---|---|---|---|
| Positive Control RNA | Total RNA from multiple cell lines, 50 ng/µl | white | 10X | 5 µl | 5 µl |
| Random Primers | Random primer mix for cDNA synthesis, 50 µM | yellow | 10X | 30 µl | 120 µl |
| PCR-Grade Water | RNase/DNase-free water for reaction mixes | white | NA | 3 ml | 12 ml |
| dNTP Mix | Mix of dATP, dGTP, dCTP and dTTP, 10 mM each | white | 20X – 50X | 95 µl | 375 µl |
| RT-EXT Buffer | cDNA synthesis and Gene-Specific primer extension reaction buffer | green | 5X | 200 µl | 780 µl |
| Reverse Transcriptase | Multiplex-optimized M-MLV Reverse Transcriptase, 200 U/µl | green | 20X | 15 µl | 55 µl |
| PCR Buffer | Reaction Buffer for 1st and 2nd PCR steps (note: tube cap says “DNA”) | red | 5X | 650 µl | 2,500 µl |
| DNA Polymerase | Multiplex-optimized thermostable DNA polymerase, 2 U/µl | red | 100X | 45 µl | 165 µl |
| Forward GS Primer Pool** | Mix of Forward Gene-Specific Primers | highlight yellow | 10X | 60 µl | 225 µl |
| Reverse GS Primer Pool** | Mix of Reverse Gene-Specific Primers | highlight yellow | 10X | 85 µl | 330 µl |
| Primer Removal Enzyme | Enzyme Mix for primer removal | orange | 150X | 15 µl | 55 µl |
| Anchor Primer Mix | Forward/Reverse Primers for 1st PCR, 2.5 mM each | yellow | 10X | 280 µl | 1,050 µl |
| Forward SeqDNA NGS Primer | Forward NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqDNA NGS Primer | Reverse NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Forward SeqIND NGS Primer | Forward NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqIND NGS Primer | Reverse NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
*The 24 reaction Assay Kit is only available for the DriverMap Human Genome-Wide Assay.
**The Forward and Reverse GS Primer Pools are specific for each kit. Please see the Product Insert for the Kit to identify which components correspond to the Primer Pools.
Box 2: Kit Components
| Component | Description | Conc. | Volume (24 reactions)* |
Volume (96 reactions) |
|---|---|---|---|---|
| Forward & Reverse Index Primer Set | Forward/Reverse Primer Pairs for PCR with Indexed Primers, pre-aliquoted in a 96-well plate | 10X | 5 µl each (24 wells)* |
5 µl each (96 wells) |
*The 24 reaction Assay Kit is only available for the DriverMap Human Genome-Wide Assay.
Kit contents should be stored at -20°C. Shelf life is 6 months.
Last modified:
12 November 2019
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