5. General Recommendations

The DriverMap AIR Controls are designed for use with PCR-based assays. For accurate results, it is essential to use Good Laboratory Practices to minimize cross-contamination of products. If possible, perform the first part of the procedure (from cDNA synthesis through the first PCR setup) in a location set aside for RNA/DNA purification work and use a set of equipment, pipettes, test tubes, and other consumables dedicated to work involving RNA/DNA. Then, run the PCR amplification reactions in a separate lab or area designated for PCR. Always change pipette tips for adding components to new samples.

For small numbers of samples (1-12), we recommend using 0.2-ml or 0.5-ml test tubes. Test tubes allow for minimal levels of cross-contamination between different samples. To minimize the hands-on time and mistakes in a liquid deposition for large numbers of samples (e.g., 24-96), we recommend running the assay in tube strips or a 96-well plate using an 8-channel (or 12-channel) pipette. After adding all the necessary reagents, seal the plate well with Clear Adhesive Film and use a Compression Pad to minimize evaporation from experimental samples. Only use each Clear Adhesive Film once. Do not reuse them.