Cellecta, Inc.
DriverMap™ Single-cell AIR Immunophenotyping Assay (Human) - V2
Table of Contents
Cellecta, Inc.
DriverMap™ Single-cell AIR Immunophenotyping Assay (Human) - V2
1.
DriverMap™ Single-cell AIR Immunophenotyping Assay – V2
2.
DriverMap™ scAIR TCR/BCR Immunophenotyping Kit Components
2.1.
DriverMap™ scAIR TCR-Mark36 Immunophenotyping Kit Components
2.2.
DriverMap™ scAIR BCR-Mark30 Immunophenotyping Kit Components
3.
Additional Materials Required
4.
General Procedure
4.1.
Workflow Outline
4.2.
Experiment Planning Guidelines
4.3.
General Procedural Guidelines
5.
Sorting T and B Cells
5.1.
Protocol for Sorting T and B cells
5.2.
Using scAIR Positive Control RNA
6.
DriverMap™ scAIR Protocol
6.1.
Hybridization with Rev-GSP Primers
6.2.
cDNA Synthesis
6.3.
Forward GSP Extension
6.4.
First PCR with Anchor Primers
6.5.
Second PCR with Indexed Primers
7.
NGS Prep for Sequencing
7.1.
QC, Quantify and Combine Samples for NGS
7.2.
Purification & Quantification of Amplified Indexed Library
7.3.
Next Generation Sequencing of Pooled Amplified Indexed Libraries
8.
Data Processing and Analysis
8.1.
Extract T- and B- Cell receptor repertoire workflow
8.2.
Convert Sequencing Data to FASTQ Format
8.3.
NGS Data QC
8.4.
Alignment and clonotypes assembly using MiXCR
8.5.
Advance Statistical Analysis
8.6.
Alignment Using the RSEM Software Package
9.
Appendices
9.1.
Appendix A. Tips and Troubleshooting
9.2.
Appendix B. Structure of Amplified Indexed Library
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
9.4.
Appendix D. List of Barcodes in the 96-well Plate
9.5.
Appendix E. List of T/B Cell Marker Genes
10.
Technical Support
11.
Contact Us
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4.
General Procedure
Last modified:
14 May 2025
3.
Additional Materials Required
4.1.
Workflow Outline
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