4.4. CRISPRtest_GBlue Data (GFP/BFP)

The following is an example of data generated from a CRISPRtest_GB experiment in Neuroblastoma Cas9 cells.

Flow Cytometry Scatter

Figure. CRISPRtest_GB experimental data, showing flow cytometry scatter plot analysis of GFP and BFP expressing cells at Day 10 in (A) Parental and (B) Cas9-expressing Neuroblastoma cells. The percentages of BFP-positive cells calculated in the GFP(-) BFP(+) quadrant Q3 and GFP-positive cells in the GFP(+) BFP(-) quadrant Q1 are used to calculate the percent knockout in the table below.

Calculations

Calculate %KO using [(GFP:BFP)Cas9 / (GFP:BFP)Parental] as follows:

1 – (0.12 / 1.25) = 90%