Use of the CRISPRtest assay requires access to a flow cytometer with the following excitation and emission requirements:

For the standard CRISPRtest Assay:
bq. Excitation: 488nm and 561nm
Emission: 530/20nm and 590/20nm

For the CRISPRtest_blue Assay:
bq. Excitation: ~450 nm (TagBFP2)
Emission: 530/20nm and 590/20nm

Cellecta also offers the following products that may be useful when running this assay:

Cas9-Only Plasmids

Cat.# Description Quantity Price
SVC9B-PS CRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (plasmid) 25 µg $300
SVC9-PS CRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (plasmid) 25 µg $300
SVC9G-PS CRISPR Cas9 pRCCG-CMV-Cas9-2A-TagGFP2 (plasmid) 25 µg $300
SVC9R-PS CRISPR Cas9 pRCCR-CMV-Cas9-2A-TagRFP (plasmid) 25 µg $300
SVC9P-PS CRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (plasmid) 25 µg $300
SVC9B-VS CRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (virus) 1 × 106 TU $500
SVC9-VS CRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (virus) 1 × 106 TU $500
SVC9G-VS CRISPR Cas9 pRCCG-CMV-Cas9-2A-TagGFP2 (virus) 1 × 106 TU $500
SVC9R-VS CRISPR Cas9 pRCCR-CMV-Cas9-2A-TagRFP (virus) 1 × 106 TU $500
SVC9P-VS CRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (virus) 1 × 106 TU $500

Positive Control Cell Line
Cas9+ MDA-MB-231 Cells may be run in parallel with your target cells as a positive control for the assay.

Cat.# Description Quantity Price
ZMDAMB231-C9 Cas9+ MDA-MB-231 Cells with Blast selection 1 × 106 cells $1,450

Transduction Control Virus
Non-Targeting CRISPR Control pRSG16-U6-(sg)-UbiC-TagRFP-2A-Puro (virus) may be used to assess transduction efficiency of the target cells.

Cat.# Description Quantity Price
SGCTL-NT-pRSG16-V Non-Targeting CRISPR control
pRSG16-U6-(sg)-UbiC-TagRFP-Puro Vector (virus)
1 × 107 TU $500

Other than the specific reagents and instruments listed above, the protocols assume the user has access to standard materials (e.g., polypropylene tubes, pipette tips), equipment (table top centrifuges, pipettes, scales), and common reagents (e.g., TE buffer, ethanol) and buffers used in a typical life science laboratory.

Last modified: Jun 13, 2020

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