Cellecta, Inc.
DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2
Table of Contents
Cellecta, Inc.
DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2
1.
DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay – V2
2.
DriverMap™ scAIR TCR/BCR Chain Pairing Kit Components
2.1.
scAIR BCR Chain Pairing Kit Components (Human/Mouse)
2.2.
scAIR TCR Chain Pairing Kit Components (Human/Mouse)
2.3.
scAIR BCR Chain Pairing Kit (Hybrid, Human-Mouse)
2.4.
scAIR TCR Chain Pairing Kit (Hybrid, Human-Mouse)
3.
Additional Materials Required
4.
General Procedure
4.1.
Workflow Outline
4.2.
Experiment Planning Guidelines
4.3.
General Procedural Guidelines
5.
Sorting T and B Cells
5.1.
Protocol for Sorting T and B cells
5.2.
Using scAIR Positive Control RNA
6.
DriverMap™ scAIR Protocol
6.1.
Hybridization with Rev-GSP Primers
6.2.
cDNA Synthesis
6.3.
Forward GSP Extension
6.4.
First PCR with Anchor Primers
6.5.
Second PCR with Indexed Primers
7.
NGS Prep for Sequencing
7.1.
QC, Quantify and Combine Samples for NGS
7.2.
Purification & Quantification of Amplified Indexed Library
7.3.
Next Generation Sequencing of Pooled Amplified Indexed Libraries
8.
Data Processing and Analysis
8.1.
Extract T- and B- Cell Receptor Chain Pairing Workflow
8.2.
Convert Sequencing Data to FASTQ Format
8.3.
NGS Data QC
8.4.
Alignment and clonotypes assembly using MiXCR
8.5.
Identification of Chain Pairs
9.
Appendices
9.1.
Appendix A. Tips and Troubleshooting
9.2.
Appendix B. Structure of Amplified Indexed Library
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
9.4.
Appendix D. List of Barcodes in the 96-well Plate
10.
Technical Support
11.
Contact Us
Download as PDF
4.
General Procedure
Last modified:
14 May 2025
3.
Additional Materials Required
4.1.
Workflow Outline
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