Cellecta, Inc.
DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2
Table of Contents
Cellecta, Inc.
  • DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2
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CELLECTA User Manual
  • 1. DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay – V2
  • 2. DriverMap™ scAIR TCR/BCR Chain Pairing Kit Components
    • 2.1. scAIR BCR Chain Pairing Kit Components (Human/Mouse)
    • 2.2. scAIR TCR Chain Pairing Kit Components (Human/Mouse)
    • 2.3. scAIR BCR Chain Pairing Kit (Hybrid, Human-Mouse)
    • 2.4. scAIR TCR Chain Pairing Kit (Hybrid, Human-Mouse)
  • 3. Additional Materials Required
  • 4. General Procedure
    • 4.1. Workflow Outline
    • 4.2. Experiment Planning Guidelines
    • 4.3. General Procedural Guidelines
  • 5. Sorting T and B Cells
    • 5.1. Protocol for Sorting T and B cells
    • 5.2. Using scAIR Positive Control RNA
  • 6. DriverMap™ scAIR Protocol
    • 6.1. Hybridization with Rev-GSP Primers
    • 6.2. cDNA Synthesis
    • 6.3. Forward GSP Extension
    • 6.4. First PCR with Anchor Primers
    • 6.5. Second PCR with Indexed Primers
  • 7. NGS Prep for Sequencing
    • 7.1. QC, Quantify and Combine Samples for NGS
    • 7.2. Purification & Quantification of Amplified Indexed Library
    • 7.3. Next Generation Sequencing of Pooled Amplified Indexed Libraries
  • 8. Data Processing and Analysis
    • 8.1. Extract T- and B- Cell Receptor Chain Pairing Workflow
    • 8.2. Convert Sequencing Data to FASTQ Format
    • 8.3. NGS Data QC
    • 8.4. Alignment and clonotypes assembly using MiXCR
    • 8.5. Identification of Chain Pairs
  • 9. Appendices
    • 9.1. Appendix A. Tips and Troubleshooting
    • 9.2. Appendix B. Structure of Amplified Indexed Library
    • 9.3. Appendix C. Obtaining and Installing Software for Data Analysis
    • 9.4. Appendix D. List of Barcodes in the 96-well Plate
  • 10. Technical Support
  • 11. Contact Us
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9. Appendices

Appendix A. Tips and Troubleshooting
Appendix B. Structure of Amplified Indexed Library
Appendix C. Obtaining and Installing Software for Data Analysis
Appendix D. List of Barcodes in the 96-well Plate

Last modified: 15 May 2025
8.5. Identification of Chain Pairs
9.1. Appendix A. Tips and Troubleshooting

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