Box 1: Kit Components
| Component | Description | Cap Color | Conc | Total Volume |
|---|---|---|---|---|
| Forward GSP TCR primer mix (human/mouse) | Mix of forward gene-specific primers to amplify the CDR1-2-3 region of TRA and TRB | n/a | 20X | 40 µl |
| scAIR TCR Positive Control RNA (human/mouse) | Total RNA from CD3+ T cells isolated from normal donor PBMC or mouse spleen (Strain CD1) | White | 20 pg/µl (human) or 50 pg/µl (mouse) | 100 μl |
| SPRI bead Dilution Buffer | Buffer to dilute SPRIselect beads two-fold prior to use | n/a | 1X | 3 ml |
| Water | Milli-Q purified water to prepare reaction mixes | White | NA | 2 ml |
| dNTP mix | Mix of dATP, dGTP, dCTP and dTTP, 10mM each | White | 20X to 50X | 80 µl |
| RT-EXT Buffer | cDNA synthesis and Forward gene-specific primer extension reaction buffer | Green | 5X | 250 µl |
| Reverse Transcriptase | Multiplex-optimized M-MLV Reverse Transcriptase (RT) | Green | 20X | 25 µl |
| Hot-Start RT Aptamer | Aptamer which inactivates RT at room temperature but loses inhibition activity at 50°C | Green | 20X | 25 µl |
| PCR Buffer | Reaction Buffer for 1st and 2nd PCR steps | Red | 5X | 600 µl |
| DNA polymerase | Multiplex-optimized thermostable hot-start DNA polymerase | Red | 100X | 35 µl |
| Anchor Primer Mix | Forward and Reverse Anchor-specific primers (AP1 and AP2) for 1st PCR | Red | 10X | 250 µl |
| Primer Removal Enzyme | Enzyme mix for primer removal | Orange | 25X | 50 µl |
| Forward SeqDNA NGS Primer | Forward SeqDNA NGS Primer for cDNA sequencing | Blue | 100 µM | 50 µl |
| Reverse SeqDNA NGS Primer | Reverse SeqDNA NGS Primer for cDNA sequencing | Blue | 100 µM | 50 µl |
| Forward SeqIND NGS Primer | Forward SeqIND NGS Primer for cDNA sequencing | Blue | 100 µM | 50 µl |
| Reverse SeqIND NGS Primer | Reverse SeqIND NGS Primer for cDNA sequencing | Blue | 100 µM | 50 µl |
Box 2: Kit Components
| Component | Description | Conc | Total Volume |
|---|---|---|---|
| scAIR TCR-Rev GSP Master Plate (human/mouse) | 96-well plate, each well contains 50µl of hybridization buffer and a mix of barcoded (with well-specific barcodes and VBC barcodes), reverse gene-specific primers designed for the C region of TRA and TRB mRNAs | 1X | 50 µl/well in a 96-well plate |
Extra Materials Required: (Sold Separately as Catalog # DM-NGS-SetA-B2, DM-NGS-SetB-B2, DM-NGS-SetC-B2, DM-NGS-SetD-B2)
| Component | Description | Conc | Total Volume |
|---|---|---|---|
| Dual Index Primer Set Plate | 96 Dual DNA/RNA UD Indexes primer pairs, pre-aliquoted, one plate sufficient to process 96 plates | 10X | 20 µl/well |
Kit contents should be stored at -20°C. Shelf life is 1 year from the date of receipt.
Last modified:
10 June 2026
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