Cellecta, Inc.
DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) - V3
Table of Contents
Cellecta, Inc.
  • DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) - V3
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. DriverMap™ EXP Human Genome-wide 19K Profiling Kit – V3
  • 2. Kit Components
  • 3. Additional Materials Required
  • 4. General Procedural Recommendations
  • 5. Sample Preparation
    • 5.1. General RNA Preparation Protocol
    • 5.2. DirectCell™ Protocol
  • 6. Protocol Outline
    • 6.1. Hybridization of mRNA with Reverse Gene-Specific Primers
    • 6.2. cDNA Synthesis
    • 6.3. Forward Gene-Specific Primer Extension
    • 6.4. First PCR with Anchor Primers
    • 6.5. Second PCR with Indexed Primers
  • 7. NGS Prep and Sequencing
    • 7.1. QC, Quantify and Combine Samples for NGS
    • 7.2. Purification of Amplified Indexed Library
    • 7.3. Next Generation Sequencing
  • 8. Data Processing and Analysis
    • 8.1. Salmon Software Package
    • 8.2. DriverMap EXP hGW19k Amplicon Sequences
  • 9. Appendices
    • 9.1. Appendix A. Tips and Troubleshooting
    • 9.2. Appendix B. Structure of Amplified Indexed Library
    • 9.3. Appendix C. List of Dual DNA/RNA UD Indexes
  • 10. Technical Support
  • 11. Contact Us
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8.2. DriverMap EXP hGW19k Amplicon Sequences

Download: Master Amplicon List

*Note: For optimal assay performance, some housekeeping genes with very high expression levels and highly homologous sequences were excluded from the gene list, including globins, ribosomal proteins, and histones.

Last modified: 3 September 2024
8.1. Salmon Software Package
9. Appendices

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