This kit contains enough reagents for the preparation of 24 or 96 NGS samples starting from total RNA isolated from blood, tissue, cells, or other biological samples or cell extracts.
Box 1: Kit Components for DriverMap™ EXP hGW19K
| Component | Description | Cap Color | Conc. | Volume (24 rxn) | Volume (96 rxn) |
|---|---|---|---|---|---|
| Forward hGW19K primer mix | Mix of forward gene-specific primers (Fwd GSP) for 19K human genes, 5X | n/a | 5X | 220 µl | 880 µl |
| Reverse hGW19K primer mix | Mix of reverse gene-specific primers (Rev GSP) for 19K human genes, 5X | n/a | 5X | 110 µl | 440 µl |
| Positive Control RNA | Total universal RNA (mix of total RNA isolated from several human tissues) | white | 50 ng/µl | 5 µl | 5 µl |
| Hybridization Buffer | Buffer for hybridization of Rev GSPs with mRNA, 4X | yellow | 4X | 1 ml | 1 ml |
| Water | Milli-Q purified water for reaction mixes | white | NA | 3 ml | 12 ml |
| dNTP Mix | Mix of dATP, dGTP, dCTP and dTTP | white | 10 mM | 100 µl | 400 µl |
| RT-EXT Buffer | cDNA synthesis and forward gene-specific primer extension reaction buffer, 5X | green | 5X | 250 µl | 1 ml |
| Reverse Transcriptase | M-MLV Reverse Transcriptase (RT) optimized for multiplex RT-PCR assay | green | 20X | 30 µl | 120 µl |
| Hot-start RT Aptamer | Aptamer which inactivates RT at room temperature but activates RT at 50°C | green | 20X | 30 µl | 120 µl |
| PCR Buffer | Reaction Buffer for 1st and 2nd PCR steps, 5X | red | 5X | 600 µl | 2 × 1.2 ml |
| DNA Polymerase | Multiplex-optimized thermostable hot-start DNA polymerase | red | 100X | 50 µl | 200 µl |
| Anchor Primer Mix | Forward/Reverse Anchor-specific Primers for 1st PCR, 10X | red | 10X | 300 µl | 1.25 ml |
| Primer Removal Enzyme | Enzyme Mix for primer removal | orange | 20-50X | 80 µl | 320 µl |
| Forward SeqDNA NGS Primer | Forward NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqDNA NGS Primer | Reverse NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Forward SeqIND NGS Primer | Forward NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqIND NGS Primer | Reverse NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
Box 2: Kit Components
| Component | Description | Conc | Volume (24 reactions) | Volume (96 reactions) |
|---|---|---|---|---|
| Dual Index Primer Set Plate | 96 Dual DNA/RNA UD Indexes primer pairs, pre-aliquoted and dried in all wells of 96-well plate | N/A | Dried 96 well plate | Dried 96 well plate |
Kit contents should be stored at -20°C. Shelf life is 1 year from date of receipt.
Last modified:
5 March 2025
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