Cellecta, Inc.
DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) - V3
Table of Contents
Cellecta, Inc.
DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) - V3
1.
DriverMap™ EXP Human Genome-wide 19K Profiling Kit – V3
2.
Kit Components
3.
Additional Materials Required
4.
General Procedural Recommendations
5.
Sample Preparation
5.1.
General RNA Preparation Protocol
5.2.
DirectCell™ Protocol
6.
Protocol Outline
6.1.
Hybridization of mRNA with Reverse Gene-Specific Primers
6.2.
cDNA Synthesis
6.3.
Forward Gene-Specific Primer Extension
6.4.
First PCR with Anchor Primers
6.5.
Second PCR with Indexed Primers
7.
NGS Prep and Sequencing
7.1.
QC, Quantify and Combine Samples for NGS
7.2.
Purification of Amplified Indexed Library
7.3.
Next Generation Sequencing
8.
Data Processing and Analysis
8.1.
Salmon Software Package
8.2.
DriverMap EXP hGW19k Amplicon Sequences
9.
Appendices
9.1.
Appendix A. Tips and Troubleshooting
9.2.
Appendix B. Structure of Amplified Indexed Library
9.3.
Appendix C. List of Dual DNA/RNA UD Indexes
10.
Technical Support
11.
Contact Us
Download as PDF
6.
Protocol Outline
Last modified:
27 February 2025
5.2.
DirectCell™ Protocol
6.1.
Hybridization of mRNA with Reverse Gene-Specific Primers
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