Cellecta, Inc.

2. Kit Components

The DriverMap™ hGW19K Blood Microsamples Expression Profiling Kit contains enough reagents for the preparation of 24 or 96 NGS samples starting from total RNA isolated from dried blood microsamples collected by Mitra tips. You can obtain Neoteryx Mitra tips for collecting 30 µl of whole blood from the Neoteryx Website. See the Additional Materials Required section for details.

The kits are shipped in two separate packages: one at room temperature and the other on blue ice or dry ice. Please store each component according to the specified temperatures listed in the tables below.

Box 1: Kit Components for RNA purification from Dried Blood Microsamples (store at +4°C):

Component Description Storage temp Cap Color Conc. Volume (24 rxn) Volume (96 rxn)
RNA Extraction Buffer Buffer for extraction of RNA from blood microsamples dried in Mitra tips +4°C n/a NA 6 ml 24 ml
Magnetic Beads Magnetic beads for binding RNA extracted from dried blood +4°C n/a NA 60 µl 240 µl
MB Wash Buffer Wash buffer for magnetic beads +4°C n/a NA 50 ml 200 ml

Box 1: Kit Components for hGW19K Assay (store at -20°C):

Component Description Storage temp Cap Color Conc. Volume (24 rxn) Volume (96 rxn)
Forward hGW19K primer mix Mix of forward gene-specific primers (Fwd GSP) for 19K human genes, 5X -20°C n/a 5X 220 µl 880 µl
Reverse hGW19K primer mix Mix of reverse gene-specific primers (Rev GSP) for 19K human genes, 5X -20°C n/a 5X 110 µl 440 µl
Proteinase K Component of RNA Extraction Master Mix -20°C n/a 25X 260 µl 1050 µl
Positive Control RNA Total RNA isolated from human PBMC blood -20°C white 50 ng/µl 5 µl 5 µl
Hybridization Buffer Buffer to elute RNA from magnetic beads and hybridize Rev GSPs with mRNA, 4X -20°C yellow 4X 1 ml 1 ml
Water Milli-Q purified water for reaction mixes -20°C white NA 3 ml 12 ml
dNTP Mix Mix of dATP, dGTP, dCTP and dTTP -20°C white 10 mM 100 µl 400 µl
RT-EXT Buffer cDNA synthesis and forward gene-specific primer extension reaction buffer, 5X -20°C green 5X 250 µl 1 ml
Reverse Transcriptase M-MLV Reverse Transcriptase (RT) optimized for multiplex RT-PCR assay -20°C green 20X 30 µl 120 µl
Hot-start RT Aptamer Aptamer which inactivates RT at room temperature but activates RT at 50°C -20°C green 20X 30 µl 120 µl
PCR Buffer Reaction Buffer for 1st and 2nd PCR steps, 5X -20°C red 5X 600 µl 2 × 1.2 ml
DNA Polymerase Multiplex-optimized thermostable hot-start DNA polymerase -20°C red 100X 50 µl 200 µl
Anchor Primer Mix Forward/Reverse Anchor-specific Primers for 1st PCR, 10X -20°C red 10X 300 µl 1.25 ml
Primer Removal Enzyme Enzyme Mix for primer removal -20°C orange 20-50X 80 µl 320 µl
Forward SeqDNA NGS Primer Forward NGS Primer for cDNA sequencing -20°C blue 100 µM 30 µl 90 µl
Reverse SeqDNA NGS Primer Reverse NGS Primer for cDNA sequencing -20°C blue 100 µM 30 µl 90 µl
Forward SeqIND NGS Primer Forward NGS Primer for Index sequencing -20°C blue 100 µM 30 µl 90 µl
Reverse SeqIND NGS Primer Reverse NGS Primer for Index sequencing -20°C blue 100 µM 30 µl 90 µl

Box 2: Kit Components for Index Primers (store at -20°C):

Component Description Storage temp Cap Color Conc. Volume (24 rxn) Volume (96 rxn)
Dual Index Primer Set Plate 96 Dual DNA/RNA UD Indexes primer pairs, pre-aliquoted and dried in all wells of 96-well plate N/A Dried 96 well plate Dried 96 well plate

Kit contents should be stored at +4°C (Box 1) and -20°C (Boxes 2 and 3). Shelf life is 1 year from date of receipt.

Last modified: 5 March 2025

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