Cellecta, Inc.
DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) for Dried Blood Microsamples - V3
Table of Contents
Cellecta, Inc.
  • DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) for Dried Blood Microsamples - V3
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. DriverMap™ EXP Human Genome-wide 19K Dried Blood Microsamples Profiling Kit – V3
  • 2. Kit Components
  • 3. Additional Materials Required
  • 4. General Procedural Recommendations
  • 5. Protocol Outline
    • 5.1. Extracting RNA from Dried Blood Microsamples
    • 5.2. Hybridization of mRNA with Reverse Gene-Specific Primers
    • 5.3. cDNA Synthesis
    • 5.4. Forward Gene-Specific Primer Extension
    • 5.5. First PCR with Anchor Primers
    • 5.6. Second PCR with Indexed Primers
  • 6. NGS Prep and Sequencing
    • 6.1. QC, Quantify and Combine Samples for NGS
    • 6.2. Purification of Amplified Indexed Library
    • 6.3. Next Generation Sequencing
  • 7. Data Processing and Analysis
    • 7.1. Salmon Software Package
    • 7.2. DriverMap EXP hGW19k Amplicon Sequences
  • 8. Appendices
    • 8.1. Appendix A. Tips and Troubleshooting
    • 8.2. Appendix B. Structure of Amplified Indexed Library
    • 8.3. Appendix C. List of Dual DNA/RNA UD Indexes
  • 9. Technical Support
  • 10. Contact Us
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10. Contact Us

Email Addresses

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Technical Support: tech@cellecta.com

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Phone Numbers

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Last modified: 24 February 2025
9. Technical Support

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