DriverMap™ AIR TCR/BCR DNA Profiling Kit contains enough reagents to prepare NGS samples starting from genomic DNA purified from blood, tissue, cells, or other biological samples, including Positive Control DNA. AIR TCR and AIR BCR kits have the same common reagent set but different TCR-specific or BCR-specific primer sets.
Box 1: Kit Components
| Component | Description | Cap Color | Concentration | Volume (24 rxn) * | Volume (96 rxn) |
|---|---|---|---|---|---|
| Reverse TCR-J primer mix (AIR TCR kit) | Mix of reverse gene-specific primers for J-region of TRA, TRB, TRG and TRD | n/a | 20X | 80 µl | 280 µl |
| Reverse BCR-J primer mix (AIR BCR kit) | Mix of reverse gene-specific primers for J-region of IGH, IGL and IGK | n/a | 20X | 80 µl | 280 µl |
| Forward TCR-FR3 primer mix (AIR TCR kit) | Mix of forward gene-specific primers for FR3-region of TRA, TRB, TRG and TRD | n/a | 20X | 80 µl | 280 µl |
| Forward BCR-FR3 primer mix (AIR BCR kit) | Mix of forward gene-specific primers for FR3-region of IGH, IGL and IGK | n/a | 20X | 80 µl | 280 µl |
| Positive Control DNA | Total PBMC genDNA (single donor) | white | 250 ng/µl | 20 µl | 40 µl |
| Water | MilliQ purified, water for reaction mixes | white | NA | 3 ml | 12 ml |
| dNTP Mix | Mix of dATP, dGTP, dCTP and dTTP | white | 10mM each | 100 µl | 400 µl |
| EXT Buffer | Buffer for Reverse and forward gene-specific primer extension steps, 5X | green | 4X | 400 µl | 1.6 ml |
| PCR Buffer | Reaction Buffer for 1st and 2nd PCR steps, 5X | red | 5X | 600 µl | 2×1.2 ml |
| DNA Polymerase | Multiplex-optimized thermostable hot-start DNA polymerase | red | 100X | 60 µl | 200 µl |
| Anchor Primer Mix | Forward/Reverse Anchor-specific Primers for 1st PCR, 10X | red | 10X | 300 µl | 1.2 ml |
| Primer Removal Enzyme | Enzyme Mix for primer removal | orange | 25-50X | 140 µl | 560 µl |
| Forward SeqDNA NGS Primer | Forward NGS Primer for DNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqDNA NGS Primer | Reverse NGS Primer for DNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Forward SeqIND NGS Primer | Forward NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqIND NGS Primer | Reverse NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
Box 2: Kit Components
| Component | Description | Conc | Volume (24 reactions) * | Volume (96 reactions) |
|---|---|---|---|---|
| Dual Index Primer Set Plate | 96 Dual DNA/RNA UD Indexes primer pairs, pre-aliquoted and dried in a 96-well plate | N/A | Dried One 96 well plate | Dried Two 96 well plates |
Kit contents should be stored at -20°C. Shelf life is 1 year.
Last modified:
23 February 2023
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