Cellecta, Inc.
DriverMap™ AIR TCR-BCR Profiling Kit (Human DNA)
Table of Contents
Cellecta, Inc.
  • DriverMap™ AIR TCR-BCR Profiling Kit (Human DNA)
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. DriverMap™ AIR DNA Profiling Assay
  • 2. DriverMap™ AIR TCR-BCR Kit Components
  • 3. Additional Materials Required
  • 4. General Procedural Recommendations
  • 5. Sample Preparation
    • 5.1. General DNA Preparation Protocol
    • 5.2. DirectCell™ Protocol
    • 5.3. General Notes
    • 5.4. FFPE Guidelines
  • 6. Outline of DriverMap™ AIR DNA Protocol
    • 6.1. Reverse J Gene-Specific (GS) Primers Extension
    • 6.2. Forward FR3 Gene-Specific (GS) Primer Extension
    • 6.3. First PCR with Anchor Primers
    • 6.4. Second PCR with Indexed Primers
  • 7. NGS Prep and Sequencing
    • 7.1. Quantify and Combine Samples for NGS
    • 7.2. Purification & Quantification of Amplified Indexed Library
    • 7.3. Next Generation Sequencing
  • 8. Data Processing and Analysis
    • 8.1. Extract T- and B- Cell receptor repertoire workflow
    • 8.2. Convert Sequencing Data to FASTQ Format
    • 8.3. NGS Data QC
    • 8.4. Alignment and clonotypes assembly using MiXCR
    • 8.5. Advance Statistical Analysis
  • 9. Appendices
    • 9.1. Appendix A. Tips and Troubleshooting
    • 9.2. Appendix B. Structure of Amplified Indexed Library
    • 9.3. Appendix C. Obtaining and Installing Software for Data Analysis
    • 9.4. Appendix D. List of Dual DNA/RNA UD Indexes
  • 10. Frequently Asked Questions
  • 11. Technical Support
  • 12. Contact Us
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9. Appendices

Appendix A. Tips and Troubleshooting
Appendix B. Structure of Amplified Indexed Library
Appendix C. Obtaining and Installing Software for Data Analysis
Appendix D. List of Dual DNA/RNA UD Indexes

Last modified: 27 January 2023
8.5. Advance Statistical Analysis
9.1. Appendix A. Tips and Troubleshooting

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