Cellecta, Inc.
DriverMap™ AIR TCR-BCR Profiling Kit (Human DNA)
Table of Contents
Cellecta, Inc.
DriverMap™ AIR TCR-BCR Profiling Kit (Human DNA)
1.
DriverMap™ AIR DNA Profiling Assay
2.
DriverMap™ AIR TCR-BCR Kit Components
3.
Additional Materials Required
4.
General Procedural Recommendations
5.
Sample Preparation
5.1.
General DNA Preparation Protocol
5.2.
DirectCell™ Protocol
5.3.
General Notes
5.4.
FFPE Guidelines
6.
Outline of DriverMap™ AIR DNA Protocol
6.1.
Reverse J Gene-Specific (GS) Primers Extension
6.2.
Forward FR3 Gene-Specific (GS) Primer Extension
6.3.
First PCR with Anchor Primers
6.4.
Second PCR with Indexed Primers
7.
NGS Prep and Sequencing
7.1.
Quantify and Combine Samples for NGS
7.2.
Purification & Quantification of Amplified Indexed Library
7.3.
Next Generation Sequencing
8.
Data Processing and Analysis
8.1.
Extract T- and B- Cell receptor repertoire workflow
8.2.
Convert Sequencing Data to FASTQ Format
8.3.
NGS Data QC
8.4.
Alignment and clonotypes assembly using MiXCR
8.5.
Advance Statistical Analysis
9.
Appendices
9.1.
Appendix A. Tips and Troubleshooting
9.2.
Appendix B. Structure of Amplified Indexed Library
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
9.4.
Appendix D. List of Dual DNA/RNA UD Indexes
10.
Frequently Asked Questions
11.
Technical Support
12.
Contact Us
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9.2.
Appendix B. Structure of Amplified Indexed Library
Last modified:
27 January 2023
9.1.
Appendix A. Tips and Troubleshooting
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
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