DriverMap™ AIR TCR-BCR RNA Profiling Kit contains enough reagents to prepare NGS samples starting from total RNA from blood, tissue, cells, or other biological samples, including Positive Control RNA. AIR TCR and BCR kits have the same reagents but different TCR or BCR-specific primer sets.
Box 1: Kit Components
| Component | Description | Cap Color | Conc. | Volume (24 rxn)* | Volume (96 rxn) |
|---|---|---|---|---|---|
| Reverse TCR-C primer mix | Mix of reverse gene-specific primers for C-region of TRA, TRB, TRG and TRD | n/a | 20X | 30 µl | 120 µl |
| Reverse BCR- C primer mix | Mix of reverse gene-specific primers for C-region of IGH, IGL and IGK | n/a | 20X | 30 µl | 120 µl |
| Forward TCR-FR1 primer mix | Mix of forward gene-specific primers for FR1-region of TRA, TRB, TRG and TRD | n/a | 20X | 60 µl | 240 µl |
| Forward BCR-FR1 primer mix | Mix of forward gene-specific primers for FR1-region of IGH, IGL and IGK | n/a | 20X | 60 µl | 240 µl |
| Positive Control RNA | Total PBMC RNA (single donor) | white | 50 ng/µl | 5 µl | 5 µl |
| Hybridization Buffer | RNA-RevGSP Hybridization Buffer, 4X | yellow | 4X | 1 ml | 1 ml |
| Water | Milli-Q purified, water for reaction mixes | white | NA | 3 ml | 12 ml |
| dNTP Mix | Mix of dATP, dGTP, dCTP and dTTP each | white | 10 mM | 125 µl | 600 µl |
| RT-EXT Buffer | cDNA synthesis and forward gene-specific primer extension reaction buffer, 5X | green | 5X | 250 µl | 1 ml |
| Reverse Transcriptase | Multiplex-optimized M-MLV Reverse Transcriptase (RT) | green | 20X | 30 µl | 120 µl |
| Hot-start RT Aptamer | Aptamer which inactivates RT at room temperature but activates RT at 50°C | green | 20X | 30 µl | 120 µl |
| PCR Buffer | Reaction Buffer for 1st and 2nd PCR steps, 5X | red | 5X | 600 µl | 2×1.2 ml |
| DNA Polymerase | Multiplex-optimized thermostable hot-start DNA polymerase | red | 100X | 50 µl | 200 µl |
| Anchor Primer Mix | Forward/Reverse Anchor-specific Primers for 1st PCR, 10X | red | 10X | 300 µl | 1.25 ml |
| Primer Removal Enzyme | Enzyme Mix for primer removal | orange | 20X – 50X | 90 µl | 360 µl |
| Forward SeqDNA NGS Primer | Forward NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqDNA NGS Primer | Reverse NGS Primer for cDNA sequencing | blue | 100 µM | 30 µl | 90 µl |
| Forward SeqIND NGS Primer | Forward NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
| Reverse SeqIND NGS Primer | Reverse NGS Primer for Index sequencing | blue | 100 µM | 30 µl | 90 µl |
Box 2: Kit Components
| Component | Description | Conc | Volume (24 reactions) * | Volume (96 reactions) |
|---|---|---|---|---|
| Dual Index Primer Set Plate | 96 Dual DNA/RNA UD Indexes primer pairs, pre-aliquoted and dried in all wells of 96-well plate | N/A | Dried 96 well plate | Dried 96 well plate |
Kit contents should be stored at -20°C. Shelf life is 1 year.
Last modified:
5 March 2025
Need more help with this?
Contact Us