The build your custom kit, you can choose from the following promoter options:
| Construct | Promoter | Volume |
|---|---|---|
| Human EF1alpha full-length promoter | EF1L | 0.5 ml |
| Human EF1alpha short promoter | EFS | 0.5 ml |
| Human cytomegalovirus promoter | CMV | 0.5 ml |
| Human cytomegalovirus promoter/enhancer | E/CMV | 0.5 ml |
| Mouse cytomegalovirus promoter | mCMV | 0.5 ml |
| Spleen focus forming virus promoter | SFFV | 0.5 ml |
| Rous sarcoma virus promoter | RSV | 0.5 ml |
| Human ubiquitin C promoter | UBC | 0.5 ml |
| Human phosphoglycerate kinase 1 promoter | PGK | 0.5 ml |
| Simian virus 40 promoter | SV40 | 0.5 ml |
Other reagents, such as cell culture media appropriate for the cell lines of interest is required, and LentiTrans™ Transduction Reagent (Cat.# LTDR1) is recommended for transduction.
Generally, the user is assumed to have access to standard materials (e.g., tissue culture plates, polypropylene tubes, pipette tips), equipment (tabletop centrifuges, pipettes, scales), and common reagents (e.g., TE buffer, ethanol) and buffers used in a typical life science laboratory.
The PromoterTest Kit should be stored at -80°C until ready for use.
Last modified:
7 February 2023
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