Box 1 Components
| Description | Concentration | Quantity |
|---|---|---|
| Taq DNA Polymerase | 50X | 200 μl (2 vials) |
| Taq DNA Polymerase Buffer | 10X | 1,200 μl (2 vials) |
| dNTP Mix | 50X (10 mM each) | 200 μl |
| PCR-Grade Water | NA | 7,320 μl (4 vials) |
Box 2 Components
| Description (Generic primer names: see Note below) |
Concentration | Quantity |
|---|---|---|
| Forward Primer | 10 μM | 150 μl |
| Reverse Primer | 10 μM | 150 μl |
| NFwd Primer | 20X (10 μM) | 240 μl |
| NRev Index Primer A | 20X (10 μM) | 20 μl |
| NRev Index Primer B | 20X (10 μM) | 20 μl |
| NRev Index Primer C | 20X (10 μM) | 20 μl |
| NRev Index Primer D | 20X (10 μM) | 20 μl |
| NRev Index Primer E | 20X (10 μM) | 20 μl |
| NRev Index Primer F | 20X (10 μM) | 20 μl |
| NRev Index Primer G | 20X (10 μM) | 20 μl |
| NRev Index Primer H | 20X (10 μM) | 20 μl |
| NRev Index Primer I | 20X (10 μM) | 20 μl |
| NRev Index Primer J | 20X (10 μM) | 20 μl |
| NRev Index Primer K | 20X (10 μM) | 20 μl |
| NRev Index Primer L | 20X (10 μM) | 20 μl |
| Seq NGS Primer* | 100 μM | 40 μl |
| Index NGS Primer | 100 μM | 40 μl |
* In the NGS Prep Kits for CRISPR-Barcode and Dual-sgRNA Libraries, two unique Seq NGS Primers are included and are required for sequencing.
Note: The generic names for each of the primers are listed above for reference. However, each version of the NGS Prep Kit has primers specific for the vector of the target library. These specific primers names also include a suffix to indicate the vector or library they target (e.g., Forward-G in LNGS-120, Forward-12 in LNGS-101, NRev-16 in LNGS-102, etc.). This Manual uses the generic primer name when, for example, it is describing which one to include in a reaction. For each generic primer reference, you should use the appropriate fully named specific primer included in your kit.
Storage Conditions:
Store kits and all components at -20°C until ready for use.
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