Possible reasons:
The Agilent Bioanalyzer is not a good tool to analyze these preps. The sizing is not as accurate as a 3% agarose gel for small fragments, and the preps tend to have a significant amount of genomic DNA which overloads the tiny Agilent capillary.
Solutions:
Use a 3% agarose gel to analyze your PCR products.
Last modified:
16 March 2017
Need more help with this?
Don’t hesitate to contact us here.