Cellecta, Inc.
Packaging, Titering, and Transduction of Lentiviral Constructs
Table of Contents
Cellecta, Inc.
  • Packaging, Titering, and Transduction of Lentiviral Constructs
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. Lentiviral Construct Packaging, Titering, and Transduction
  • 2. Materials Provided
    • 2.1. Additional Materials for Production of Lentivirus
    • 2.2. Additional Materials for Transduction of Cells
  • 3. Recommended Experiments to Characterize Target Cells
    • 3.1. Cell Doubling Time
    • 3.2. Calculating a Kill Curve
    • 3.3. Check Toxicity of LentiTrans Transduction Reagent
  • 4. General Protocols for Lentiviral Packaging and Transduction
    • 4.1. General Lentiviral Packaging Protocol
    • 4.2. General Lentiviral Transduction Protocol
    • 4.3. Assessing Transduction Efficiency by Flow Cytometry or Antibiotic Selection
    • 4.4. Lentiviral Titer Calculation
  • 5. Technical Support
  • 6. Safety Guidelines
  • 7. Contact Us
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2. Materials Provided

  • Lentiviral expression vector or construct from Cellecta: cDNA or InDOXible vector, or other
  • Recommended:
    • LentiPrep™ Reagent Set (Cat.# LTSET-G or LTSET-R, includes the 3 items listed below)
    • Ready-to-Use Lentiviral Packaging Plasmid Mix (Cellecta, Cat.# CPCP-K2A)
    • LentiTrans™ Transduction Reagent, 1000x (Cellecta, Cat.# LTDR1)
    • LentiFuge™ Viral Concentration Reagent (Cellecta, Cat.# LFVC1)
Last modified: 10 February 2020
1. Lentiviral Construct Packaging, Titering, and Transduction
2.1. Additional Materials for Production of Lentivirus

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