Cellecta, Inc.
Packaging, Titering, and Transduction of Lentiviral Constructs
Table of Contents
Cellecta, Inc.
  • Packaging, Titering, and Transduction of Lentiviral Constructs
GrabGrab
GrabGrab
CELLECTA User Manual
  • 1. Lentiviral Construct Packaging, Titering, and Transduction
  • 2. Materials Provided
    • 2.1. Additional Materials for Production of Lentivirus
    • 2.2. Additional Materials for Transduction of Cells
  • 3. Recommended Experiments to Characterize Target Cells
    • 3.1. Cell Doubling Time
    • 3.2. Calculating a Kill Curve
    • 3.3. Check Toxicity of LentiTrans Transduction Reagent
  • 4. General Protocols for Lentiviral Packaging and Transduction
    • 4.1. General Lentiviral Packaging Protocol
    • 4.2. General Lentiviral Transduction Protocol
    • 4.3. Assessing Transduction Efficiency by Flow Cytometry or Antibiotic Selection
    • 4.4. Lentiviral Titer Calculation
  • 5. Technical Support
  • 6. Safety Guidelines
  • 7. Contact Us
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4. General Protocols for Lentiviral Packaging and Transduction

This section provides key protocols for packaging, transducing, and titering lentiviral plasmid libraries and constructs.

Last modified: 10 January 2019
3.3. Check Toxicity of LentiTrans Transduction Reagent
4.1. General Lentiviral Packaging Protocol

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