Most of the lentiviral vectors used in making Cellecta’s libraries express an antibiotic resistance marker. Regardless of the antibiotic selection marker of the library, you need to know the concentration of antibiotic that kills non-transduced cells within a given amount of time in order to successfully select transduced cells. In order to avoid the enrichment of double integrants and loss of single-integrants, we recommend avoiding over-selection: calculate an antibiotic kill curve in your cell line beforehand and choose the lowest antibiotic concentration which gives 90%-95% killing. For fine-tuning of the antibiotic concentration, if the lentivirus carries both an antibiotic selection marker and a fluorescence marker, follow the procedure described in the Antibiotic Selection of Library Transduced Cells section.
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