4.2. No sgRNA/Barcodes Present in NGS Results

Possible reason #1:

Incorrect First or Second Round PCR Primers or Sequencing Primer used.

Solution:

Ensure that you use the correct First and Second Round PCR Primers and Cellecta Sequencing Primer for your library. Check the Library Cassette Diagram provided with the kit.

Possible reason #2:

Incorrect NGS reagent kit used.

Solution:

Ensure that you or your NGS Core Facility uses the proper Illumina NGS reagent kit. For details, please check the kit descriptions in the Additional Materials Required section.