Use of the CRISPRuTest assay requires access to a flow cytometer with the following excitation and emission requirements:
Excitation: 488nm and 561nm
Emission: 530/20nm and 590/20nm
Cellecta also offers the following products that may be useful when running this assay:
Cas9-Only Plasmids
| Cat.# | Description | Quantity |
|---|---|---|
| SVC9B-PS | CRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (plasmid) | 25 µg |
| SVC9-PS | CRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (plasmid) | 25 µg |
| SVC9G-PS | CRISPR Cas9 pRCCG-CMV-Cas9-2A-TagGFP2 (plasmid) | 25 µg |
| SVC9R-PS | CRISPR Cas9 pRCCR-CMV-Cas9-2A-TagRFP (plasmid) | 25 µg |
| SVC9P-PS | CRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (plasmid) | 25 µg |
| SVC9B-VS | CRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (virus) | 1 × 106 TU |
| SVC9-VS | CRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (virus) | 1 × 106 TU |
| SVC9G-VS | CRISPR Cas9 pRCCG-CMV-Cas9-2A-TagGFP2 (virus) | 1 × 106 TU |
| SVC9R-VS | CRISPR Cas9 pRCCR-CMV-Cas9-2A-TagRFP (virus) | 1 × 106 TU |
| SVC9P-VS | CRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (virus) | 1 × 106 TU |
Additional Cas9-Only plasmids are available. Please visit our CRISPR Cas9 Expression Construct web page.
Positive Control Cell Line
Cas9+ MDA-MB-231 Cells may be run in parallel with your target cells as a positive control for the assay.
| Cat.# | Description | Quantity |
|---|---|---|
| ZMDAMB231-C9 | Cas9+ MDA-MB-231 Cells with Blast selection | 1 × 106 cells |
Transduction Control Virus
Non-Targeting CRISPR Control pRSG16-U6-sg-UbiC-TagRFP-2A-Puro (virus) may be used to assess transduction efficiency of the target cells.
| Cat.# | Description | Quantity |
|---|---|---|
| SGCTL-NT-pRSG16-V | Non-Targeting CRISPR control pRSG16-U6-sg-UbiC-TagRFP-Puro Vector (virus) |
1 × 107 TU |
Non-targeting controls in other Cellecta vectors are available. Please visit our CRISPR Non-Targeting sgRNA Control Construct in Standard Lentiviral Vector web page.
Other than the specific reagents and instruments listed above, the protocols assume the user has access to standard materials (e.g., polypropylene tubes, pipette tips), equipment (table top centrifuges, pipettes, scales), and common reagents (e.g., TE buffer, ethanol) and buffers used in a typical life science laboratory.
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