Transcriptional repression efficiency of in the dCas9-Rep cell line is calculated as the ratio between the normalized GFP intensity of Parental Cells and the normalized GFP intensity of dCas9-Rep Cells.
The data above was calculated using the suggested positive control MDA-MB-231-dCas9-KRAB and parental MDA-MB-231 cells (see Additional Required Materials). The level of repression is calculated as follows using this data as an example:
| Mean_GFP | Mean_RFP | GFP/RFP | |
|---|---|---|---|
| Parental | 334 | 291 | 334 / 291 = 1.148 |
| dCas9-KRAB | 27 | 221 | 27 / 221 = 0.122 |
dCas9-Rep fold repression = 1.148 / 0.122 = 9.4 fold
Last modified:
10 January 2019
Need more help with this?
Contact Us