Cellecta’s pre-made and custom CRISPR Pooled Lentiviral sgRNA Libraries are provided either as plasmid DNA or as packaged VSV-G pseudotyped lentiviral particles. The pre-made human genome-wide pooled CRISPR sgRNA Knockout Libraries are available in two formats—as a single module or as three modules. Both library options cover all human protein-coding genes.
Single-Module CRISPR Genome-Wide Knockout Libraries
Genome-wide coverage in single-module, pooled human and mouse libraries that can be conveniently used for a wide variety of screening approaches.
| Human Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-RFP-Puro (plasmid) | 79,268 | KOHGW-80Kv2-P | 200 µg | -20°C |
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-RFP-Puro (virus) | 79,268 | KOHGW-80Kv2-V8 | 2×108 TU | -80°C |
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-RFP-Puro (virus) | 79,268 | KOHGW-80Kv2-V9 | 1×109 TU | -80°C |
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-GFP-Puro (plasmid) | 79,268 | KOHGW-UGP-80Kv2-P | 200 µg | -20°C |
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-GFP-Puro (virus) | 79,268 | KOHGW-UGP-80Kv2-V8 | 2×108 TU | -80°C |
| CRISPR Human Genome 80K v2 Knockout Library in UbiC-GFP-Puro (virus) | 79,268 | KOHGW-UGP-80Kv2-V9 | 1×109 TU | -80°C |
| Mouse Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| CRISPR Mouse Genome 86K Knockout Library in UbiC-RFP-Puro (plasmid) | 86,241 | KOMGW-86K-P | 200 µg | -20°C |
| CRISPR Mouse Genome 86K Knockout Library in UbiC-RFP-Puro (virus) | 86,241 | KOMGW-86K-V8 | 2×108 TU | -80°C |
| CRISPR Mouse Genome 86K Knockout Library in UbiC-RFP-Puro (virus) | 86,241 | KOMGW-86K-V9 | 1×109 TU | -80°C |
For information on previous library versions (e.g. KOHGW-80K, KOMGW-80K, and KOMGW-80Kv2), please contact us at info@cellecta.com.
Three-Module CRISPR Human Genome-Wide 150K Knockout Library
Deep coverage of 8 sgRNA per target gene divided into 3 modules that may be combined or used separately, depending on the type of screen desired.
| Three-Module Human Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| CRISPR Human Genome Knockout Library, Module 1 in UbiC-RFP-Puro (plasmid) | 52,549 | KOHGW-M1-P | 200 µg | -20°C |
| CRISPR Human Genome Knockout Library, Module 2 in UbiC-RFP-Puro (plasmid) | 49,461 | KOHGW-M2-P | 200 µg | -20°C |
| CRISPR Human Genome Knockout Library, Module 3 in UbiC-RFP-Puro (plasmid) | 48,578 | KOHGW-M3-P | 200 µg | -20°C |
| CRISPR Human Genome Knockout Library, Modules 1, 2, & 3 in UbiC-RFP-Puro (plasmid) | 150,588 Total | KOHGW-M123-P | 200 µg each | -20°C |
| CRISPR Human Genome Knockout Library, Module 1 in UbiC-RFP-Puro (virus) | 52,549 | KOHGW-M1-V8 | 2×108 TU | -80°C |
| CRISPR Human Genome Knockout Library, Module 2 in UbiC-RFP-Puro (virus) | 49,461 | KOHGW-M2-V8 | 2×108 TU | -80°C |
| CRISPR Human Genome Knockout Library, Module 3 in UbiC-RFP-Puro (virus) | 48,578 | KOHGW-M3-V8 | 2×108 TU | -80°C |
| CRISPR Human Genome Knockout Library, Module 1 in UbiC-RFP-Puro (virus) | 52,549 | KOHGW-M1-V9 | 1×109 TU | -80°C |
| CRISPR Human Genome Knockout Library, Module 2 in UbiC-RFP-Puro (virus) | 49,461 | KOHGW-M2-V9 | 1×109 TU | -80°C |
| CRISPR Human Genome Knockout Library, Module 3 in UbiC-RFP-Puro (virus) | 48,578 | KOHGW-M3-V9 | 1×109 TU | -80°C |
CRISPR Livestock and Poultry Genome-Wide Knockout Libraries
CRISPR Chicken and Pig Genome-Wide Knockout Libraries for convenient, genome-wide dropout viability screens. CRISPR screening of livestock and poultry could be useful in the study of pathogen resistance, improved breeding, and more.
| Livestock and Poultry Genome-Wide Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| CRISPR Chicken Genome Knockout Library in UbiC-RFP-Puro (plasmid) | 69,343 | KOCGW-69K-P | 200 µg | -20°C |
| CRISPR Chicken Genome Knockout Library in UbiC-RFP-Puro (virus) | 69,343 | KOCGW-69K-V8 | 2×108 TU | -80°C |
| CRISPR Chicken Genome Knockout Library in UbiC-RFP-Puro (virus) | 69,343 | KOCGW-69K-V9 | 1×109 TU | -80°C |
| CRISPR Pig Genome Knockout Library in UbiC-RFP-Puro (plasmid) | 89,217 | KOPGW-89K-P | 200 µg | -20°C |
| CRISPR Pig Genome Knockout Library in UbiC-RFP-Puro (virus) | 89,217 | KOPGW-89K-V8 | 2×108 TU | -80°C |
| CRISPR Pig Genome Knockout Library in UbiC-RFP-Puro (virus) | 89,217 | KOPGW-89K-V9 | 1×109 TU | -80°C |
CRISP-Seq Barcoded CRISPR Knockout sgRNA Libraries (Perturb-Seq, CROP-Seq)
Pooled lentiviral libraries with a barcoded sgRNA expression cassette that is expressed and can be detected using RNA Sequencing. Run CRISPR screens with single-cell RNA-Sequencing platforms (aka Perturb-Seq or CRISP‑Seq).
| CRISP-Seq Barcoded Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| CloneTracker XP™ Human 27 Anti-Cancer CRISPR-Barcode-3’ Library – RFP-Puro (plasmid) | 560 | KOBCXPL-HAC27-P | 200 µg | -20°C |
| CloneTracker XP™ Human 27 Anti-Cancer CRISPR-Barcode-3’ Library – RFP-Puro (virus) | 560 | KOBCXPL-HAC27-V | 1×108 TU | -80°C |
| CloneTracker XP™ Mouse 27 Anti-Cancer CRISPR-Barcode-3’ Library – RFP-Puro (plasmid) | 559 | KOBCXPL-MAC27-P | 200 µg | -20°C |
| CloneTracker XP™ Mouse 27 Anti-Cancer CRISPR-Barcode-3’ Library – RFP-Puro (virus) | 559 | KOBCXPL-MAC27-V | 1×108 TU | -80°C |
Single-Module Genome-Wide CRISPRa and CRISPRi Libraries
| Human CRISPRa/i Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| Human Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (plasmid) | 106,400 | KAHGW-106-P | 200 µg | -20°C |
| Human Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (virus) | 106,400 | KAHGW-106-V8 | 2×108 TU | -80°C |
| Human Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (virus) | 106,400 | KAHGW-106-V9 | 1×109 TU | -80°C |
| Human Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (plasmid) | 106,386 | KIHGW-106-P | 200 µg | -20°C |
| Human Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (virus) | 106,386 | KIHGW-106-V8 | 2×108 TU | -80°C |
| Human Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (virus) | 106,386 | KIHGW-106-V9 | 1×109 TU | -80°C |
| Mouse CRISPRa/i Libraries | # sgRNA | Catalog # | Amount | Storage |
|---|---|---|---|---|
| Mouse Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (plasmid) | 109,186 | KAMGW-109-P | 200 µg | -20°C |
| Mouse Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (virus) | 109,186 | KAMGW-109-V8 | 2×108 TU | -80°C |
| Mouse Genome-Wide CRISPRa sgRNA Library in UbiC-RFP-Puro (virus) | 109,186 | KAMGW-109-V9 | 1×109 TU | -80°C |
| Mouse Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (plasmid) | 109,505 | KIMGW-109-P | 200 µg | -20°C |
| Mouse Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (virus) | 109,505 | KIMGW-109-V8 | 2×108 TU | -80°C |
| Mouse Genome-Wide CRISPRi sgRNA Library in UbiC-RFP-Puro (virus) | 109,505 | KIMGW-109-V9 | 1×109 TU | -80°C |
Single-Module Genome-Wide CRISPRa and CRISPRi Dual-sgRNA Libraries
| Human CRISPRa/i Dual-sgRNA Libraries | # sgRNA pairs | Catalog # | Amount | Storage |
|---|---|---|---|---|
| Human Genome-Wide CRISPRa Dual-sgRNA Library in UbiC-RFP-Puro (plasmid) | 105,130 | KADHGW-105K-P | 200 µg | -20°C |
| Human Genome-Wide CRISPRa Dual-sgRNA Library in UbiC-RFP-Puro (virus) | 105,130 | KADHGW-105K-V8 | 2×108 TU | -80°C |
| Human Genome-Wide CRISPRa Dual-sgRNA Library in UbiC-RFP-Puro (virus) | 105,130 | KADHGW-105K-V9 | 1×109 TU | -80°C |
| Human Genome-Wide CRISPRi Dual-sgRNA Library in UbiC-RFP-Puro (plasmid) | 105,135 | KIDHGW-105K-P | 200 µg | -20°C |
| Human Genome-Wide CRISPRi Dual-sgRNA Library in UbiC-RFP-Puro (virus) | 105,135 | KIDHGW-105K-V8 | 2×108 TU | -80°C |
| Human Genome-Wide CRISPRi Dual-sgRNA Library in UbiC-RFP-Puro (virus) | 105,135 | KIDHGW-105K-V9 | 1×109 TU | -80°C |
Product Information
For information on the specific CRISPR knockout, CRISPRa, or CRISPRi sgRNA Library that you have purchased, please review the product information you received by email upon delivery (either by link or attachment):
- Product Certificate (PC or PAC)
- The Product Certificate contains important information such as the viral titer and volume (for libraries provided as viral particles), complexity, mutation rate, clone sequencing QC data, NGS QC data, and Lot Number.
- sgRNA Sequence Files
- Provided as a Microsoft Excel file upon purchase, the sequence file contains target gene names, sgRNA sequences, clone sequencing data, and NGS QC data including reads, distribution, complexity or number of independent clones, and mutation rate. If you have not received it, please contact us at tech@cellecta.com.
- NGS Cassette Diagram
- Available in PDF format, the NGS cassette diagram shows the sgRNA sequence structure, design of sgRNA expression cassette including location of PCR and NGS primers, and primer sequences. The NGS cassettes of Cellecta’s standard sgRNA vectors are available on the Cloning and Expression Vector Information web page.
- Vector Map and Sequence
- sgRNA library vector sequences are available in SnapGene, GenBank/VectorNTI (.gb), and FASTA formats. The sequences of Cellecta’s standard sgRNA vectors are available on the Cloning and Expression Vector Information web page.
- Alignment Software Supporting Files
- The Cellecta LNGS Prep Kits include the Alignment Software which is used to demultiplex and deconvolute Illumina NGS data. Supporting files such as the Library Configuration Files (.json format) and Sample Description Form are available on the respective CRISPR Knockout Library Product Pages and CRISPRa/i Library Product Pages
Additional information on Cellecta’s pre-made libraries can be found on the following web pages:
- CRISPR Human Genome-Wide Knockout Libraries
- CRISPR Mouse Genome-Wide Knockout Library
- CRISPRa and CRISPRi Human Genome-Wide Libraries
- CRISPRa and CRISPRi Mouse Genome-Wide Libraries
- Dual-CRISPRa and Dual-CRISPRi Genome-Wide Libraries
Last modified:
30 April 2025
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