CloneTracker™ XP 10-Barcode Cell Labeling Kit
(Cat.# BCXP3RPM10-V)
Box 1 (Reagents for amplification and sequencing)
Contains reagents for amplification and sequencing of up to 10 genomic DNA samples.
Storage: -20°C
Component Description Conc. Volume Taq DNA Polymerase Thermostable DNA Polymerase 50X 300 µl Taq DNA Polymerase Buffer Reaction Buffer for PCR 10X 1,500 µl dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each 50X 300 µl PCR-Grade Water Water for Reaction Mixes NA 10 ml First PCR Primer: Forward-XP First Round Forward PCR Primer (10 uM) 20X 150 µl First PCR Primer: Reverse-XP First Round Reverse PCR Primer (10 uM) 20X 150 µl Second PCR Primer: NFwd-XP Second Round Forward PCR Primer (10 uM) 20X 240 µl Second PCR Primer: NRev-XP Index A Second Round Reverse PCR Primer (10 uM) 20X 20 µl Positive Control Template DNA Positive Control template—genomic DNA labeled with one unique, single-copy barcode (0.2 µg/µl) 10X 25 µl
Box 2 (Pre-packaged lentiviral particles)
Panel of 10 packaged barcode labeling constructs; >1×107 TU of each construct.
Storage: -80°C
CloneTracker™ 5-Barcode Cell Labeling Kit
(Cat.# BCRPP5-V)
Box 1 (Reagents for amplification and sequencing)
Contains reagents for amplification and sequencing of up to 10 genomic DNA samples.
Storage: -20°C
Component Description Conc. Volume Taq DNA Polymerase Thermostable DNA Polymerase 50X 300 µl Taq DNA Polymerase Buffer Reaction Buffer for PCR 10X 1,500 µl dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each 50X 300 µl PCR-Grade Water Water for Reaction Mixes NA 10 ml First PCR Primer Mix Mix of Forward and Reverse PCR Primers for First PCR (2.5 µM each) 10X 120 µl Second PCR Primer Mix Mix of Forward and Reverse PCR Primers for Second PCR (2.5 µM each) 10X 1,500 µl Positive Control Template DNA Positive Control template—genomic DNA labeled with one unique, single-copy barcode (0.2 µg/µl) 10X 25 µl
Box 2 (Pre-packaged lentiviral particles)
Panel of 5 packaged barcode labeling constructs; >1×107 TU of each construct.
Storage: -80°C
Barcodes are the same as the first 5 barcodes in the 10-Barcode Cell Labeling Kit. Constructs have both fluorescent (RFP) and puromycin resistance markers (pRSI16-U6-bc-UbiC-TagRFP-2A-Puro).
CloneTracker™ 10-Barcode Cell Labeling Kit
(Cat.# BCRPP10-V)
Box 1 (Reagents for amplification and sequencing)
Contains reagents for amplification and sequencing of up to 10 genomic DNA samples.
Storage: -20°C
Component Description Conc. Volume Taq DNA Polymerase Thermostable DNA Polymerase 50X 300 µl Taq DNA Polymerase Buffer Reaction Buffer for PCR 10X 1,500 µl dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each 50X 300 µl PCR-Grade Water Water for Reaction Mixes NA 10 ml First PCR Primer Mix Mix of Forward and Reverse PCR Primers for First PCR (2.5 µM each) 10X 120 µl Second PCR Primer Mix Mix of Forward and Reverse PCR Primers for Second PCR (2.5 µM each) 10X 1,500 µl Positive Control Template DNA Positive Control template—genomic DNA labeled with one unique, single-copy barcode (0.2 µg/µl) 10X 25 µl
Box 2 (Pre-packaged lentiviral particles)
Panel of 10 packaged barcode labeling constructs; >1×107 TU of each construct.
Storage: -80°C
The first 5 barcodes are the same ones in the 5-Barcode Cell Labeling Kit. Constructs have both fluorescent (RFP) and puromycin resistance markers (pRSI16-U6-bc-UbiC-TagRFP-2A-Puro).
CloneTracker™ 4-Barcode-Plus Cell Labeling Kit
(Cat.# BC4P-V)
Box 1 (Reagents for amplification and sequencing)
Contains reagents for amplification and sequencing of up to 20 genomic DNA samples.
Storage: -20°C
Component Description Conc. Volume Taq DNA Polymerase Thermostable DNA Polymerase 50X 50 µl Taq DNA Polymerase Buffer Reaction Buffer for PCR 10X 500 µl dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each 50X 50 µl PCR-Grade Water Water for Reaction Mixes NA 2 ml First PCR Primer Mix Mix of Forward and Reverse PCR Primers for First PCR (2.5 µM each) 10X 120 µl Second PCR Primer Mix Mix of Forward and Reverse PCR Primers for Second PCR (2.5 µM each) 10X 1,500 µl Barcode 1 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 1 (2.5 µM each) 10X 120 µl Barcode 2 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 2 (2.5 µM each) 10X 120 µl Barcode 3 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 3 (2.5 µM each) 10X 120 µl Barcode 4 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 4 (2.5 µM each) 10X 120 µl Positive Control Template DNA Positive Control template—mix of 4 genomic DNAs (1:1 ratio) each labeled with one of the 4 Barcodes (0.2 µg/µl) 10X 25 µl
Box 2 (Pre-packaged lentiviral particles)
Panel of 4 packaged barcode labeling constructs; >1×107 TU of each construct.
Storage: -80°C
Barcodes have unique sequences and sizes, and they can be differentially amplified with primers specific to each of the barcodes. Constructs have both fluorescent (RFP) and puromycin resistance markers (pRSI16-U6-bc-UbiC-TagRFP-2A-Puro).
CloneTracker™ 4-Barcode-Plus Cell Labeling Kit (Puro only)
(Cat.# BC4PPO-V)
Box 1 (Reagents for amplification and sequencing)
Contains reagents for amplification and sequencing of up to 20 genomic DNA samples.
Storage: -20°C
Component Description Conc. Volume Taq DNA Polymerase Thermostable DNA Polymerase 50X 50 µl Taq DNA Polymerase Buffer Reaction Buffer for PCR 10X 500 µl dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each 50X 50 µl PCR-Grade Water Water for Reaction Mixes NA 2 ml First PCR Primer Mix Mix of Forward and Reverse PCR Primers for First PCR (2.5 µM each) 10X 120 µl Second PCR Primer Mix Mix of Forward and Reverse PCR Primers for Second PCR (2.5 µM each) 10X 1,500 µl Barcode 1 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 1 (2.5 µM each) 10X 120 µl Barcode 2 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 2 (2.5 µM each) 10X 120 µl Barcode 3 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 3 (2.5 µM each) 10X 120 µl Barcode 4 PCR Primer Mix Mix of Forward and Reverse PCR Primers specific for Barcode 4 (2.5 µM each) 10X 120 µl Positive Control Template DNA Positive Control template—mix of 4 genomic DNAs (1:1 ratio) each labeled with one of the 4 Barcodes (0.2 µg/µl) 10X 25 µl
Box 2 (Pre-packaged lentiviral particles)
Panel of 4 packaged barcode labeling constructs; >1×107 TU of each construct.
Storage: -80°C
Barcodes have unique sequences and sizes, and they can be differentially amplified with primers specific to each of the barcodes. Constructs have a puromycin-resistance marker, but no fluorescent marker (pRSIUP-U6-bc-UbiC-Puro).
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