Cellecta, Inc.
cDNA and InDOXible Cloning and Expression Vector User Manual
Table of Contents
Cellecta, Inc.
  • cDNA and InDOXible Cloning and Expression Vector User Manual
GrabGrab
GrabGrab
CELLECTA User Manual
  • cDNA and InDOXible Cloning and Expression Vectors
  • Materials Provided
    • Additional Materials for Cloning
    • Additional Materials for Production of Lentivirus
    • Additional Materials for Transduction of Cells
  • Cloning cDNA Into Vector
    • cDNA Cloning Protocol
  • Recommended Experiments to Characterize Target Cells
    • Cell Doubling Time
    • Calculating a Kill Curve
    • Check Toxicity of LentiTrans Transduction Reagent
  • General Protocols for Lentiviral Packaging and Transduction
    • General Lentiviral Packaging Protocol
    • General Lentiviral Transduction Protocol
    • Assessing Transduction Efficiency by Flow Cytometry or Antibiotic Selection
    • Lentiviral Titer Calculation
  • Technical Support
  • Safety Guidelines
  • Contact Us
Download as PDF

Materials Provided

  • Lentiviral expression vector or construct from Cellecta: cDNA or InDOXible vector, or other
  • Recommended:
    • LentiPrep™ Reagent Set (Cat.# LTSET-G or LTSET-R, includes the 3 items listed below)
    • Ready-to-Use Lentiviral Packaging Plasmid Mix (Cellecta, Cat.# CPCP-K2A)
    • LentiTrans™ Transduction Reagent, 1000x (Cellecta, Cat.# LTDR1)
    • LentiFuge™ Viral Concentration Reagent (Cellecta, Cat.# LFVC1)
cDNA and InDOXible Cloning and Expression Vectors
Additional Materials for Cloning

Need more help with this?
Contact Us

Thanks for your feedback.
Copyright (c) 2025 Cellecta, Inc.