- We recommend using the Qiagen All Prep DNA/RNA FFPE kit. With FFPE the yield and quality of isolated DNA/RNA can vary significantly, depending on the age and method used to fix the sample.
- Certain impurities and high concentrations of FFPE-DNA can inhibit DNA polymerase activity for AIR-DNA profiling assay. To identify the optimal FFPE-DNA input amount, we suggest testing a range of DNA inputs (e.g., 0.5 µg, 1 µg, 2 µg, 5 µg) for at least one sample (e.g., sample with a high yield of purified DNA), in our experience 1-2 µg can be used for AIR-DNA assay without inhibition of DNA polymerase activity.
- For more information, please refer to our Technology Guide.
Last modified:
6 February 2026
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