5. Sample Preparation

Depending on the sample amount, either total RNA or isolated immune cells (i.e., DirectCell Protocol) can be used as input for the DriverMap AIR Assay. No preliminary poly(A) selection or ribosomal/mitochondrial/globin RNA depletion is required:

• For bulk tissue, blood, or cell samples with more than ~50,000 cells, follow the RNA Preparation Protocol.
• For biological samples with a small number of cells—less than ~50,000 cells (e.g., sorted immune cells), or blood and tissue microsamples—we recommend using the immune cells directly as input. Follow the DirectCell Protocol.