7. NGS Prep and Sequencing

The product from the PCR with Indexed Primers step contains both P5 and P7 sequences and dual DNA/RNA UDP Indexes for Next-Gen Sequencing (NGS) on Illumina instruments. The protocol in this section provides the instructions to normalize the amount of each Amplified Indexed Library to obtain similar reads for each sample, to combine and clean up samples before loading onto the instrument, and guidelines for sequencing and data analysis.