Cellecta, Inc.
DriverMap™ AIR TCR-BCR Profiling Kit (RNA)
Table of Contents
Cellecta, Inc.
DriverMap™ AIR TCR-BCR Profiling Kit (RNA)
1.
DriverMap™ AIR RNA Profiling Assay
2.
DriverMap™ AIR TCR-BCR Profiling Kit Components
3.
Additional Materials Required
4.
General Procedural Recommendations
5.
Sample Preparation
5.1.
General Guidelines
5.2.
RNA Preparation Protocol
5.3.
DirectCell™ Protocol
6.
Outline of DriverMap™ AIR RNA Protocol
6.1.
Hybridization of mRNA with Reverse C-region Gene-Specific Primers
6.2.
cDNA Synthesis
6.3.
Forward Gene-Specific Primer Extension
6.4.
First PCR with Anchor Primers
6.5.
Second PCR with Indexed Primers
7.
NGS Prep and Sequencing
7.1.
QC, Quantify and Combine Samples for NGS
7.2.
Purification of Amplified Indexed Library
7.3.
Next Generation Sequencing
8.
Data Processing and Analysis
8.1.
Extract T- and B- Cell receptor repertoire workflow
8.2.
Convert Sequencing Data to FASTQ Format
8.3.
NGS Data QC
8.4.
Alignment and clonotypes assembly using MiXCR
8.5.
Advance Statistical Analysis
9.
Appendices
9.1.
Appendix A. Tips and Troubleshooting
9.2.
Appendix B. Structure of Amplified Indexed Library
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
9.4.
Appendix D. List of Dual DNA/RNA UDP Indexes
10.
Technical Support
11.
Contact Us
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9.2.
Appendix B. Structure of Amplified Indexed Library
Last modified:
30 September 2022
9.1.
Appendix A. Tips and Troubleshooting
9.3.
Appendix C. Obtaining and Installing Software for Data Analysis
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