Bright Texture

Pre-Processing > Bright Texture

Highlights bright components of image textures that are no bigger than the specified window size. Also known as a top-hat filter, it computes the grayscale opening (grayscale dilation followed by grayscale erosion) of the Current Image by the specified window size, then subtracts the result from the Current Image.

1. Window Size

Window size (in pixels) to consider about each pixel to calculate the filter. Controls the maximum size of bright feature components to highlight.

Tips

• Useful for increasing the contrast of flat grey scale features, for example, cells imaged under phase contrast.
• Useful for removing large overexposed regions in fluorescence imaging.
• Starting Window Size: depends on image resolution, start in the middle of the slider, increase to increase brightness, decrease to decrease brightness.